*High energy intermediates are needed to form polymers
- the amount of triphosphates limits the number added to the polymer
- a monophosphate can be used to start the chain, but then triphosphates must be used
*RNA polymerase = protein enzyme
- Adding RNA polymerase to a system containing an RNA sequence does not increase the rate of the reaction because there are no binding sites for the template
*Polynucleotides are built from 5’ to 3’
- a phosphodiester bond connects the nucleotides
- weak hydrogen bonds connect the base pairs (A,C,G,U)
*An enzyme that has binding sites for a template and for free nucleotides can rapidly generate copies of RNA
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